Gene Expression is affected when cells are cultured in petri dish

When trying to figure out how different respond to drugs and other environemnts in the body scientists turn to cells. The cultured cells are grown in petri dishes now with the onset of tissue microarrays the process is getting more advanced

A new Brown University study shows that nerve cells grown in three-dimensional cultures use 1,766 genes differently compared to cells grown in standard two-dimensional petri dishes.

The research shows that culture techniques can significantly affect cell growth and function. cells grown in a laboratory in 3-D environments are more like cells grown in the ultimate 3-D environment – the human body.

“More and more, we’re seeing evidence that cells cultured in three dimensions look and behave more like cells in your body,” said Diane Hoffman-Kim, the University of Brown bioengineer who spearheaded the new study,

The study is published in the May edition of Tissue Engineering,

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8 Responses

  1. […] When trying to figure out how different respond to drugs and other environemnts in the body scientists turn to cells. The cultured cells are grown in petri dishes now with the onset of tissue… …more […]

  2. […] When trying to figure out how different respond to drugs and other environemnts in the body scientists turn to cells. The cultured cells are grown in petri dishes now with the onset of tissue… …more […]

  3. […] When trying to figure out how different respond to drugs and other environemnts in the body scientists turn to cells. The cultured cells are grown in petri dishes now with the onset of tissue… …more […]

  4. […] When trying to figure out how different respond to drugs and other environemnts in the body scientists turn to cells. The cultured cells are grown in petri dishes now with the onset of tissue… …more […]

  5. […] When trying to figure out how different respond to drugs and other environemnts in the body scientists turn to cells. The cultured cells are grown in petri dishes now with the onset of tissue… …more […]

  6. […] When trying to figure out how different respond to drugs and other environemnts in the body scientists turn to cells. The cultured cells are grown in petri dishes now with the onset of tissue… …more […]

  7. Why do people consistently do these specious array experiments?

    All the above study shows is that cells cultured in one kind of dish are different from cells cultured in another kind of dish. Where’s the study that shows that cells cultured in their “3D” system are more like the in vivo cells? In order to do that study, they’d have to compare primary cells isolated directly from tissue with cells cultured in their system, and if they could get enough cells to do that analysis, they wouldn’t need 3D culture in the first place.

    People should be careful about overselling their results.

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